Biology question
The question is:
Which of the following has a better resolution limit?
Fluorescence microscopy (green light, 530 nm), air objective (n = 1.0) with an NA = 1.21
Fluorescence micrsocopy (red light, 605 nm), oil objective (n=1.3), with an NA = 1.41
Fluorescence microscopy (far-red light, 655 nm), oil objective (n=1.3) with an NA = 1.45
How do I begin to solve the question? Cuz the objective lens is different from the NA how do I take that into account?
October 4, 2014
2 Comments • Newest first
I'd say just plug it into the resolution equation? I don't think objective matters, but I could be wrong.
I know resolution is proportional to wavelength/NA... but your medium (i.e. oil/air) is factored into NA, so I don't think you need both values.
I checked my notes and we used D = (0.61lambda)/(Nsinalpha) for our cell bio course (Nsinalpha is equal to NA).
pls help aznseal ):